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At the family level (Figure 5B), 217 families were found among all soil samples, and the dominant families (top 6) were Burkholderiaceae (7.0851.31%), Sphingomonadaceae (1.7114.84%), Gemmatimonadaceae (2.2613.10%), Caulobacteraceae (2.378.63%), Xanthobacteraceae (1.786.67%), Rhodanobacteraceae (1.215.67%), etc

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Reverse transcription was performed using a PrimeScript RT reagent kit with gDNA eraser (Takara) following the manufacturers instructions